Knowledge Base

If  you  encounter  an  access  violation  (something  like:  “Access  to  the  path
“C:\WINDOWS\Microsoft.NET\Framework\v2.0.50727\Temporary ASP.NET
Files is denied…”) the reason is the following:
On  Windows  Server  2003  the  default  account  ASP.NET  runs  under  is  the
process account ‘NETWORK SERVICE’ (of WWW services), not the ASPNET
user.  So  in  other  words,  you  need  to  give  these  permissions  for  the
NETWORK SERVICE account.

2.  Here is a short explanation of how to grant access to the temporary files folder to a certain user account:

The elegant way to repair an ASP.NET on IIS installation is to use the aspnet_regiis.exe utility provided
within the Framework directory. There’s a huge number of options, but the one we want for this
scenario is the “-ga” option to grant a user access to the IIS metabase and the directories required
(including the temporary directory)

C:\WINDOWS\Microsoft.NET\Framework\v2.0.50727>aspnet_regiis.exe
Administration utility (2.0.50727) to install and uninstall ASP.NET on the
local machine.
Copyright (c) Microsoft Corporation. All rights reserved.

— ASP.NET REGISTRATION OPTIONS —
<…snip!…>

-ga <user>   Grant the specified user or group access to the IIS
metabase and other directories used by ASP.NET.

C:\WINDOWS\Microsoft.NET\Framework\v2.0.50727>aspnet_regiis.exe -ga “DOMAIN\USERNAME”

Related resources:

http://social.msdn.microsoft.com/Forums/en-US/asmxandxml/thread/fbc5c85c-2001-4a54-ba15-1182ebac0387